3D_Deconvolution

  When looking at a three-dimensional (3D) specimen through a transmitted brightfield optical microscope, only the part of the specimen contained in the focal plane appears sharp while the remainder looks smooth. The deconvolution task consists in deblurring the observed image in order to recover the original shapes of the object. Although 2D deconvolution softwares are available freely, the 3D consideration gives rise to many problems leading to the impossibility to recover the original images without any assumptions. Moreover some noise is added to the signal, it has been considered as a white Gaussian noise.